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Firma Nyheter:
- Effect of base modifications on structure, thermodynamic stability, and . . .
Silencing activity of siRNA duplexes directed toward EGFP mRNA (A) Sequences of two siRNA molecules, indicated as G1 and G2 (common region is underlined) (B) Silencing activity of G1 and G2 used in 1–50 nM concentrations (C) HeLa cells viability 36 h after transfection with siRNAs in concentrations given above The means ± standard deviation is given
- Effect of base modifications on structure, thermodynamic . . . - PubMed
Effect of base modifications on structure, thermodynamic stability, and gene silencing activity of short interfering RNA RNA 2007 Aug;13(8):1301-16 doi: 10 1261 rna 538907
- RNA major groove modifications improve siRNA stability and biological . . .
INTRODUCTION RNA interference (RNAi) represents a powerful molecular strategy for inhibition of gene expression ( 1–3) This process can be induced by 21–23 nt double-stranded RNAs (dsRNAs), known as short interfering RNAs (siRNAs) ( 4–6) These oligoribonucleotides (formed by a sense and an antisense strand) are recognized by the RNA-induced silencing complex (RISC) ( 7, 8), a protein
- Chemical Modification of Small Interfering RNA
The discovery of RNA interference (RNAi) by Fire et al not only triggered a paradigm change in our perception of RNA as a molecule taking active part in gene regulation, but also provided new RNA tools to experimentally reduce expression of specific genes in both basic research and therapeutics Particularly, the demonstration by Tuschl et al in 2001 that synthetic 21-mer double-stranded (ds
- Chemical strategies for strand selection in short-interfering RNAs . . .
An siRNA molecule is recognized by cytosolic Argonaute 2 (AGO2), forming the pre-RNA induced silencing complex (pre-RISC) AGO2 preferentially orients the dsRNA in the pre-RISC based on the properties of the sequence, thermodynamic stability, and structure of the 5′ ends 7,8 This orientation determines which strand of the siRNA duplex is chosen as the guide strand, and ideally selects the
- Effect of asymmetric terminal structures of short RNA duplexes on the . . .
Short interfering RNAs (siRNAs) are valuable reagents for sequence-specific inhibition of gene expression via the RNA interference (RNAi) pathway Although it has been proposed that the relative thermodynamic stability at the 5′-ends of siRNAs plays
- Effect of base modifications on structure, thermodynamic stability, and . . .
Abstract A series of nucleobase-modified siRNA duplexes containing “rare” nucleosides, 2-thiouridine (s 2 U), pseudouridine (Ψ), and dihydrouridine (D), were evaluated for their thermodynamic stability and gene silencing activity The duplexes with modified units at terminal positions exhibited similar stability as the nonmodified reference
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